A high-resolution cytogenetic map of human chromosome 12: Localization of 195 new cosmid markers by direct R-banding fluorescence in situ hybridization |
| |
Authors: | Ei-ichi Takahashi Kumiko Koyama Akitsu Hitomi Yusuke Nakamura |
| |
Institution: | (1) Division of Genetics, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, 263 Chiba, Japan;(2) Department of Biochemistry, Cancer Institute, 1-37-1 Kami-Ikebukuro, Toshima-ku, 170 Tokyo, Japan |
| |
Abstract: | We have constructed a high-resolution cytogenetic map of human chromosome 12 with 195 newly isolated cosmids by direct R-banding flourescence in situ hybridization. The fluorescent signals of 195 clones were evenly distributed throughout chromosome 12, but sublocalized preferentially to R-positive bands. This high-resolution cytogenetic map with an average map distance of 0.73 Mb on bands can, in conjunction with a genetic linkage map, facilitate the analysis of chromosomal and molecular aberrations in genetic diseases and cancers. Moreover, the cytogenetic mapping data provide starting points for establishing contig maps with cosmid clones and yeast artificial chromosomes. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|