福氏志贺菌ipaB基因的克隆及其在酵母细胞中的表达

志贺氏菌引起的细菌性痢疾为一种全球性的肠道传染病。据估计，全世界每年感染的人数超过两亿，由该病引起的死亡人数有65万左右1]。该菌的致病性是由体内含有230kb的毒性大质粒决定的，而大质粒上一个31kb的片段所编码的侵袭质粒抗原(IInvasion plasmid antigen,Ipa)是致病所必需的2,3]。近年来，国内外有关学者在原核生物中对ipaB基因克隆及功能进行了较广泛的研究4,5]，但在酵母细胞中这方面的研究未见报导。从志贺痢疾杆菌中克隆了ipaB基因，并在酵母细胞中得到了融合表达，为将IpaB应用于双杂交系统研究其在侵袭过程中的分子机制打下了基础。

Cloning of ipaB Gene from Shigella flexneri and its Expression in Yeast Cell

ipaB gene amplified from S. flexneri 2a by PCR was cloned into pGBKT7 vector (Named pGBKT-ipaB) and sequencing indicated that the amino encoded by the cloned gene was the same as the reported; The recombination plasmid pGBKT-ipaB was transformed into the yeast strain AH109, in which the fusion expression of IpaB was analyzed with SDS-PAGE and Western blotting. This provided the basis for screening the interacting protein with IpaB by two hybrid system and understanding the function of IpaB in the pathogenesis of S. flexneri further.