The analysis of urinary steroids using gas-liquid chromatography |
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| Affiliation: | 1. Office of Testing and Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, US Food and Drug Administration, St. Louis, MO 63110, USA;2. Office of Compounding Quality and Compliance, Office of Compliance, Center for Drug Evaluation and Research, US Food and Drug Administration, Silver Spring, MD 20993, USA;1. Servei d′Anàlisis Cliniques Hospital Universitari Son Espases, Palma de Mallorca, Spain;2. Bioanalysis Research Group, IMIM, Hospital del Mar, Doctor Aiguader 88, 08003 Barcelona, Spain;3. Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Doctor Aiguader 88, 08003 Barcelona, Spain;4. Doping Control Research Group, IMIM, Hospital del Mar, Doctor Aiguader 88, 08003 Barcelona, Spain;5. Integrative Pharmacology and Systems Neuroscience Research Group, IMIM (Hospital del Mar Research Institute), Dr. Aiguader 88, 08003 Barcelona, Spain;1. Research Service, Louis Stokes Cleveland VA Medical Center, Cleveland, OH, USA;2. Department of Spinal Cord Injury, Louis Stokes Cleveland VA Medical Center, Cleveland, OH, USA;3. Department of Medicine, Case Western Reserve University, Cleveland, OH, USA;4. Department of Physical Medicine and Rehabilitation, Case Western Reserve University, Cleveland, OH, USA;5. MetroHealth Research Institute, MetroHealth Medical Center, 2500 MetroHealth Drive, Cleveland, OH, USA;6. Ohio University Heritage College of Osteopathic Medicine, Cleveland, OH, USA |
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| Abstract: | A method is described using gas-liquid chromatography for the routine determination of urinary C19O2 and C19O3-17-Ketosteroids, pregnanediol, and pregnanetriol in the urine of normal subjects. Following hydrolysis, the extract is applied to thin-layer silica gel and developed in a system which allows separation of steroid metabolites into 3 zones. The steroids in each zone are measured using conditions that provide optimal separation from other metabolites and impurities. Pregnanediol is analyzed as its diacetate after preliminary thin-layer chromatography. These methods have high accuracy, speed and precision, and may be used in normal as well as abnormal states. |
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