Measurement of urinary testosterone by gas-liquid chromatography |
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| Affiliation: | 1. Faculty of Pharmacy, University of Concepción, Concepción, Chile;2. Hospital Dr. Fernando Escalante Pradilla, CCSS, San José, Costa Rica;3. Department of Pharmacology, Faculty of Biological Sciences, University of Concepción, Chile |
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| Abstract: | A method has been developed which employs gas-liquid chromatography for the assay of testosterone from extracts of human urine prepared according to an earlier method described by Camacho & Migeon in 1963. The trimethyl silyl ethers of testosterone and an internal standard, cistestosterone, were separated on the nitrile silicone rubber stationary phase XE-60. This method has given results which correspond satisfactorily with those obtained with UV spectrophotometry or measurement of sulfuric acid chromogens after paper chromatography. |
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