The determination of urinary testosterone using thin-layer chromatography and gas chromatography |
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| Affiliation: | 1. Institute of Sciences, Meritorious Autonomous University of Puebla, Puebla, Mexico;2. Faculty of Chemical Engineering, Meritorious Autonomous University of Puebla, Puebla, Mexico;1. School of Environmental Ecology and Biological Engineering, School of Chemistry and Environmental Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan 430205, PR China;2. Department of Physics, National University of Singapore, 2 Science Drive 3, 117542, Singapore;1. Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan;2. Department of Civil and Environmental Engineering, University of Delaware, Newark, USA;1. Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), S.A.S Nagar, 160062, Punjab, India;2. Aurigene Discovery Technologies Ltd., Electronics City Phase II, Bengaluru, 560100, Karnataka, India;1. Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow 119121, Russia;2. Pirogov Russian National Research Medical University, Moscow, Russia;3. Institute of Bioorganic Chemistry, Minsk, Belarus;1. Research Scholar, Department of Civil Engineering, Thapar Institute of Engineering and Technology, Patiala 147004, Punjab;2. Professor, Department of Civil Engineering, Thapar Institute of Engineering and Technology, Patiala 147004, Punjab;3. Professor, School of Chemistry and Biochemistry, Thapar Institute of Engineering and Technology, Patiala 147004, Punjab |
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| Abstract: | A new method has been devised for the relatively rapid and sensitive determination of testosterone in human urine. One fourth of a 24 hour urine sample was incubated with β-glucuronidase after addition of a tracer dose of 1,2–3H-testosterone for the calculation of recovery and extracted with ethyl ether. The crude extract was subjected to thin-layer chromatography (TLC), eluted, acetylated, subjected to TLC again and analyzed by gas chromatography. This method detected as little as 5 μg of testosterone per 24 hour and the rate of recovery was 54.8±0.4% (Mean±S.E.). The value for normal adult men ranged from 41 to 200 μg/day, showing definite decline with advancing age. While the urinary testosterone exhibits a very low level (5>-8 μg/day) in normal adult women, hirsute women excreted almost as much testosterone as normal adult men. |
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