Protein composition of the carboxysomes of Thiobacillus neapolitanus |
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Authors: | Yolande A Holthuijzen Jan F L van Breemen J Gijs Kuenen Wil N Konings |
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Institution: | (1) Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands;(2) Biochemical Laboratory, University of Groningen, Nijenborgh 16, NL-9747 AG Groningen, The Netherlands;(3) Department of Microbiology, Technical University, Julianalaan 67A, NL-2628 BC Delft, The Netherlands |
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Abstract: | For purifying carboxysomes of Thiobacillus neapolitanus an isolation procedure was developed which resulted in carboxysomes free from whole cells, protoplasts and cell fragments. These purified carboxysomes are composed of 8 proteins and at the most of 13 polypeptides. The two most abundant proteins which make up more than 60% of the carboxysomes, are ribulose-1,5-bisphosphate carboxylase and a glycoprotein with a molecular weight of 54,000. The shell of the carboxysomes consists of four glycoproteins, one also with a molecular weight of 54,000. The other proteins are present in minor quantities. Ribulose-1,5-bisphosphate carboxylase is the only enzyme which could be detected in the carboxysomes and 3-phosphoglycerate was the only product formed during incubation with ribulose-1,5-diphosphate and bicarbonate. The supernatant of a broken and centrifuged carboxysome suspension contained the large subunit of ribulose-1,5-bisphosphate carboxylase. The small subunit of ribulose-1,5-bisphosphate carboxylase was found in the pellet together with the shell proteins which indicates that the small subunit of ribulose-1,5-bisphosphate carboxylase is connected to the shell.Abbreviations RuBisCO
ribulose-1,5-bisphosphate carboxylase
- PMSF
phenylmethylsulfonyl fluoride
- PAA
gelectrophoresis, polyacrylamide gelelectrophoresis
- SDS
sodium dodecyl sulphate
- CIE
crossed immunoelectrophoresis
- IEF
isoelectric focusing |
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Keywords: | Carboxysomes Polyhedral bodies Protein composition Ribulose-1 5-bisphosphate carboxylase Shell glycoproteins |
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