Asymmetric somatic hybridization between wheat (Triticum aestivum) and Avena sativa L. |
| |
Authors: | XIANG Fengning XIA Guangmin CHEN Huimin |
| |
Institution: | (1) Life Science School, Shandong University, 250100 Jinan, China |
| |
Abstract: | Protoplasts from cell suspensions ofyoung-embryo-derived calli, which were nonregenerable for long-term subculture and protoplasts
from embryogenic calli with the regeneration capacity of 75% ofthe same wheat Jinan 177, were mixed as recipient. Protoplasts
from embryogenic calli of Avena sativa (with the regeneration capacity ofless than 10%) irradiated with UV at an intensity of 300 μW/cm2 for 30 s, 1 min, 2 min, 3 min, 5 min were used as the donor. Protoplasts ofthe recipient and the donor were fused by PEG
method. Many calli and normal green plants were regenerated at high frequency, and were verified as somatic hybrids by chromosome
counting, isozyme, 5S rDNA spacer sequence analysis and GISH (genomic in situ hybridization). Fusion combination between protoplasts either from the cell suspensions or from the calli and UV-treated
Avena sativa protoplasts could not regenerate green plants. |
| |
Keywords: | Triticum aestivum Avena sativa L UV-irradiation asymmetric fusion hybrids regenerated plant |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学:生命科学英文版》浏览原始摘要信息 |
| 点击此处可从《中国科学:生命科学英文版》下载免费的PDF全文 |