(1) Department of Experimental Physics, Faculty of Science, Palacký University, 771 46 Olomouc, Czech Republic;(2) Center for Microscopic Methods, Faculty of Medicine, Palacký University, I. P. Pavlova 35, 775 20 Olomouc, Czech Republic
Abstract:
A relative decrease of the high temperature part (above 60°C) of the chlorophyll fluorescence temperature curve during 3 h to 10 h greening period of barley (Hordeum vulgare L.) leaves was found to be concomitant to a decrease of Chl alb ratio and to a gradual increase of LHCP/core ratio found by electrophoresis and the ratio of granal to total length of thylakoid membranes. It is suggested that the high temperature part of the fluorescence temperature curve depends inversely on the relative amount of LHC II in thylakoid membranes.Abbreviations Chl a(b)
chlorophyll a(b)
- CPa
chlorophyll a protein complex of PS II
- CP1
P700 chlorophyll a protein complex of PS I
- FP
free pigments
- FTC
fluorescence temperature curve
- F(T30)
fluorescence intensity at 30°C
- LHC II
light harvesting complex II
- LHCP
light harvesting chlorophyll protein
- LHCP3 (LHCPm)
monomeric form of LHC II
- LHCPo
oligomeric form of LHC II complex
- M1
first maximum of FTC
- M2
second maximum (region) of FTC
- PAA
polyacrylamide
- PAR
photosynthetically active radiation
- PS I(II)
Photosystem I(II)
- SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis