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Immunocytochemistry of magnocellular neurons of supraoptic and paraventricular nuclei of normal and Brattleboro rats with vasopressin anti-idiotype antibody |
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| Authors: | Dr. Karl M. Knigge Diane T. Piekut David J. Berlove |
| Affiliation: | (1) The Neuroendocrine Unit, Center for Brain Research, University of Rochester, Rochester, New York, USA;(2) Neuroendocrine Unit, Box 609, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, 14642 Rochester, NY, USA |
| Abstract: | Summary A vasopressin anti-idiotype antibody was generated by immunization with purified IgG of a primary vasopressin antiserum. The anti-idiotype antibody immunostained neurons in the supraoptic and paraventricular nuclei of the hypothalamus of normal and Brattleboro rats. The distribution of immunostained perikarya in these hypothalamic nuclei together with the staining of fibers in median eminence and neural lobe was similar to that observed in normal rats with anti-vasopressin and suggests strongly that vasopressinergic neurons are being stained. Absorption studies with vasopressin and a vasopressin-binding receptor protein further indicate that a receptor associated with vasopressinergic neurons is recognized by the anti-idiotype antibody.Supported by NIH grants ES03239, NS18626 and NSF grant BNS-8310914. D.T.P. is the receipient of RCDA award NS00869 |
| Keywords: | Anti-idiotype antibody Vasopressin Immunocytochemistry Receptors Brattleboro rat |
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