Acrylamide synthesis using agar entrapped cells of <Emphasis Type="Italic">Rhodococcus rhodochrous</Emphasis> PA-34 in a partitioned fed batch reactor |
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Authors: | Jog Raj Nitya Nand Sharma Shreenath Prasad Tek Chand Bhalla |
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Institution: | (1) Department of Biotechnology, Himachal Pradesh University, Shimla, 171 005, India |
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Abstract: | The nitrile hydratase (Nhase) induced cells of Rhodococcus rhodochrous PA-34 catalyzed the conversion of acrylonitrile to acrylamide. The cells of R. rhodochrous PA-34 immobilized in 2% (w/v) agar (1.76 mg dcw/ml agar matrix) exhibited maximum Nhase activity (8.25 U/mg dcw) for conversion
of acrylonitrile to acrylamide at 10°C in the reaction mixture containing 0.1 M potassium phosphate buffer (pH 7.5), 8% (w/v)
acrylonitrile and immobilized cells equivalent to 1.12 mg dcw (dry cell weight) per ml. In a partitioned fed batch reaction
at 10°C, using 1.12 g dcw immobilized cells in a final volume of 1 l, a total of 372 g of acrylonitrile was completely hydrated
to acrylamide (498 g) in 24 h. From the above reaction mixture 87% acrylamide (432 g) was recovered through crystallization
at 4°C. By recycling the immobilized biocatalyst (six times), a total of 2,115 g acrylamide was produced. |
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Keywords: | Rhodococcus rhodochrous PA-34 Immobilized biocatalyst Agar Acrylonitrile Acrylamide Partitioned reactor |
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