WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis |
| |
| Authors: | Morisaki Tetsuro Hirota Toru Iida Shin-ichi Marumoto Tomotoshi Hara Toshihiro Nishiyama Yasuyuki Kawasuzi Michio Hiraoka Takehisa Mimori Tatsuyuki Araki Norie Izawa Ichiro Inagaki Masaki Saya Hideyuki |
| |
| Affiliation: | Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, 2-2-1 Honjo, Kumamoto 860-0811, Japan. |
| |
| Abstract: | Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
