Biotin-phenyldiazomethane conjugates as labeling reagents at phosphate in mono and polynucleotides |
| |
Authors: | Bourget Cécile Trévisiol Emmanuelle Bridon Isabelle Kotera Mitsuharu Lhomme Jean Laayoun Ali |
| |
Institution: | LEDSS-UMR5616 associé au CNRS, BP53, Université Joseph Fourier, 38041 Grenoble, France. |
| |
Abstract: | Molecules 2-5 that include in their structure a biotin moiety as detectable unit and differently substituted phenyl diazo functions as reactive group were prepared as reagents for labeling the phosphate group in mono and polynucleotides. These molecules were shown to react selectively and quantitatively with the model nucleotide 3'-UMP. They were used successfully in the labeling step of DNA and RNA analysis using high-density DNA-chips (or microarrays) technology. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|