| 表皮生长因子受体调控的人鼻咽癌细胞系CNE2分泌蛋白质的筛选 |
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| 引用本文： | 粱可,陈主初,易 红,李建玲,张鹏飞,李茂玉,李 萃,冯雪萍,彭 芳,肖志强.表皮生长因子受体调控的人鼻咽癌细胞系CNE2分泌蛋白质的筛选[J].生物化学与生物物理进展,2007,34(1):100-106. |
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| 作者姓名： | 粱可 陈主初 易 红 李建玲 张鹏飞 李茂玉 李 萃 冯雪萍 彭 芳 肖志强 |
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| 作者单位： | 1. 中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙,410008;中南大学湘雅医学院肿瘤研究所,长沙,410078
2. 中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,长沙,410008 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);教育部跨世纪优秀人才培养计划 |
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| 摘 要： | 为筛选表皮生长因子受体(epidermalgrowthfactorreceptor,EGFR)调控的鼻咽癌(nasopharyngealcarcinoma,NPC)细胞的分泌蛋白质,揭示EGFR在NPC发病中的作用机制,采用无血清培养法培养NPC细胞系CNE2,并用转化生长因子(transforminggrowthfactor-α,TGF-α)刺激CNE2细胞24h作为实验组,对照组CNE2细胞不用TGF-α刺激.超滤法脱盐并浓缩两组细胞的培养上清制备分泌蛋白,采用双向凝胶电泳技术(two-dimensionalelectrophoresis,2-DE)分离两组细胞的分泌蛋白,PDquest图像分析软件识别差异表达的蛋白质点,基质辅助激光解吸电离飞行时间质谱(matrix-assistedlaserdesorption/ionizationtimeofflightmassspectrometry,MALDI-TOF-MS)鉴定差异表达蛋白.建立了实验组和对照组CNE2细胞分泌蛋白的2-DE图谱,图像分析识别了22个差异蛋白质点,质谱鉴定了8个非冗余蛋白质,其功能涉及肿瘤细胞侵袭转移、细胞凋亡和增殖,为进一步揭示EGFR在NPC发病中的作用及其机制奠定了基础.
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| 关 键 词： | 鼻咽癌 CNE2细胞 表皮生长因子受体(EGFR) 分泌蛋白质组 二维凝胶电泳 质谱 |
| 收稿时间： | 6/21/2006 3:23:20 PM |
| 修稿时间： | 8/2/2006 5:13:01 PM |
Screening of EGFR-regulated Secreted Proteins in Human NPC Cell Line CNE2 |
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| Liang Ke,CHEN Zhu-Chu,YI Hong,LI Jian-Ling,ZHANG Peng-Fei,LI Mao-Yu,LI Cui,FENG Xue-Ping,PENG Fang and XIAO Zhi-Qiang.Screening of EGFR-regulated Secreted Proteins in Human NPC Cell Line CNE2[J].Progress In Biochemistry and Biophysics,2007,34(1):100-106. |
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| Authors: | Liang Ke CHEN Zhu-Chu YI Hong LI Jian-Ling ZHANG Peng-Fei LI Mao-Yu LI Cui FENG Xue-Ping PENG Fang and XIAO Zhi-Qiang |
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| Institution: | Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China;Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China |
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| Abstract: | |
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| Keywords: | nasopharyngeal carcinoma (NPC) CNE2 epidermal growth factor receptor (EGFR) secretome two-dimensional gel electrophoresis mass spectrometry |
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