| BLyS受体TACI、BCMA与人IgG1 Fc融合蛋白在哺乳动物细胞中的表达与鉴定 |
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| 引用本文： | 田瑞阳,黄玉辉,陈悦,韩威,俞缨,刘惠,翁忠辉,杨胜利,龚毅.BLyS受体TACI、BCMA与人IgG1 Fc融合蛋白在哺乳动物细胞中的表达与鉴定[J].动物学报,2004,50(3):401-407. |
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| 作者姓名： | 田瑞阳 黄玉辉 陈悦 韩威 俞缨 刘惠 翁忠辉 杨胜利 龚毅 |
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| 作者单位： | 中国科学院上海生命科学研究院,中国科学院上海生物工程研究中心,上海,200233 |
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| 基金项目： | “8 63”计划资助项目 (No 2 0 0 1AA2 15 3 81)~~ |
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| 摘 要： | 从人肝脏和肾脏cDNA文库中扩增了人IgG1Fc与B淋巴细胞刺激因子 (BLyS)的受体TACI及BCMA的胞外编码区 ,构建了TACI Fc及BCMA Fc融合表达质粒pSec1 Fc TACI与 pSec1 Fc BCMA ,并使用电穿孔法转染COS 7细胞 ,从 1L无血清培养上清中可纯化得到分泌表达的TACI Fc与BCMA Fc融合蛋白约 2mg。为获得TACI Fc与BCMA Fc的稳定来源 ,构建了TACI Fc与BCMA Fc的CHO稳定表达细胞株。免疫沉淀和ELISA结果显示 ,TACI Fc及BCMA Fc能特异性地结合其配体BLyS而不与BLyS同家族的TNF结合。TACI Fc及BCMA Fc可阻断BLyS对体外培养的小鼠B淋巴细胞的促增殖作用
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| 关 键 词： | BLyS TACI BCMA Fc 融合蛋白 哺乳动物细胞 |
Expression and identification of TACI/BCMA-IgG1 Fc fusion proteins in mamamlian cells |
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| TIAN Rui Yang,HUANG Yu Hu,CHEN Yue,HAN Wei,YU Ying,LIU Hui,WENG Zhong Hui,YANG Sheng Li,GONG Yi Shanghai Research Center of Biotechnology,Shanghai Institute for Biological Sciences,The Chinese Academy of Sciences,Shanghai ,China.Expression and identification of TACI/BCMA-IgG1 Fc fusion proteins in mamamlian cells[J].Acta Zoologica Sinica,2004,50(3):401-407. |
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| Authors: | TIAN Rui Yang HUANG Yu Hu CHEN Yue HAN Wei YU Ying LIU Hui WENG Zhong Hui YANG Sheng Li GONG Yi Shanghai Research Center of Biotechnology Shanghai Institute for Biological Sciences The Chinese Academy of Sciences Shanghai China |
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| Institution: | TIAN Rui Yang,HUANG Yu Hu,CHEN Yue,HAN Wei,YU Ying,LIU Hui,WENG Zhong Hui,YANG Sheng Li,GONG Yi ** Shanghai Research Center of Biotechnology,Shanghai Institute for Biological Sciences,The Chinese Academy of Sciences,Shanghai 200233,China |
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| Abstract: | The cDNA of human IgG1 Fc and the extracelluar domains of the receptors of B lymphocyte stimulator (BLyS), TACI and BCMA, were amplified from cDNA libraries of human liver and kidney. The secretory expression plasmids of TACI Fc and BCMA Fc fusion proteins were constructed and transfected to COS 7 cells by electroporation. About 2 mg of purified fusion proteins were obtained from 1 liter of serum free cell culture supernatant. Stable CHO cell lines which express TACI Fc and BCMA Fc were also constructed. Immunoprecipitation and ELISA demonstrated that TACI Fc and BCMA Fc specifically bind to BLyS but not to TNF, which belongs to the same superfamily of BLyS. The stimulation of mouse B lymphocyte triggered by BLyS can be inhibited with TACI Fc and BCMA Fc in vitro . |
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| Keywords: | BlyS TACI BCMA Fc Fusion protein Mammalian cell |
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