Acoel spermatozoa: ultrastructure and immunocytochemistry of tubulin |
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Authors: | Raikova Olga I Flyatchinskaya Ludmila P Justine Jean-Lou |
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Institution: | (1) 1Zoological Institute, 199034 St.-Petersburg, Russia;(2) 2Laboratoire de Biologie Parasitaire, Protistologie, Helminthologie, Muséum National d'Histoire Naturelle, 61 rue Buffon, F-75231 Paris cedex 05, France; E-mail |
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Abstract: | Acoel spermatozoa are filiform and contain two parallel axonemes, which do not show the trepaxonematan 9 + ‘1’ pattern, but
instead, another kind of 9 + ‘1’ pattern, or a 9 + 0 or 9 + 2 pattern. Spermatozoa have either cortical singlet microtubules
or central microtubules. Identification of these groups of microtubules and recognition of homologies between species is difficult
with electron microscopy. In addition to conventional electron microscopy, indirect immunofluorescence of tubulin was performed
on three species (Symsagittifera schultzei, Symsagittifera psammophila, and Actinoposthia beklemischevi). This technique facilitated
understanding of the general morphology of the filiform spermatozoon and of the arrangement of the microtubular organelles
along its length. We have found that different monoclonal antibodies (anti-alpha-, anti-alpha-acetylated- and anti-beta-tubulin)
can distinguish distinct subcellular populations of microtubules. The axonemes were labelled by the three antibodies in all
species. The cortical microtubules (in Actinoposthia beklemischevi) were labelled by the three antibodies. The central microtubules
(in Symsagittifera schultzei and S. psammophila) were labelled with the anti-beta-tubulin antibody and not labelled by the
anti-alpha- and anti-alpha-acetylated-tubulin. Similar experiments were performed on other Platyhelminthes and indicated that
immunocytochemistry of spermatozoa may provide new characters for phylogenetic studies.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Platyhelminthes Acoela spermatozoa immunocytochemistry tubulin microtubules |
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