| 青蒿内生真菌的抗肿瘤抗氧化活性 |
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| 引用本文： | 钱一鑫,康冀川,耿坤,王鲁,雷帮星,何珺.青蒿内生真菌的抗肿瘤抗氧化活性[J].菌物研究,2014(1):44-50. |
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| 作者姓名： | 钱一鑫 康冀川 耿坤 王鲁 雷帮星 何珺 |
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| 作者单位： | [1]贵州大学西南药用生物资源教育部工程研究中心,贵阳550025 [2]贵州大学农学院,贵阳550025 [3]贵州大学药学院,贵阳550025 [4]贵阳市植保植检站,贵阳550081 |
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| 基金项目： | 贵阳市社会发展与民生科技计划项目[筑科合同（2012103）67号],贵州省科技厅农业攻关项目[黔科合NY（2013）3042号,黔科合NY（2013）3044号],贵州省科技厅国际合作项目[黔科合外G字（2012）7006号],贵州省科技创新人才团队建设项日[黔科合人才团队（2012）4007号] |
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| 摘 要： | 采用MTT法和DPPH法,分别测定已分离得到的68株贵州青蒿内生真菌乙酸乙酯粗提物的肿瘤细胞生长抑制率和DPPH自由基清除率。试验共筛选获得12株活性内生真菌,根据其形态特征进行鉴定,分别隶属于子囊菌亚门(Ascomycotina)的链格孢属(Alternaria)、刺盘孢属(Colletotrichum)、拟盘多毛孢属(Pestalotiopsis)和拟茎点霉属(Phomopsis);其中,有8株内生真菌至少对1种指示瘤株具有细胞毒活性,占总菌株数的11.8%;5株内生真菌具有不同程度的清除DPPH自由基活性,占总菌株数的7.4%;1株内生真菌同时具有细胞毒活性和抗氧化活性。
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| 关 键 词： | 青蒿 内生真菌 细胞毒活性 抗氧化活性 形态鉴定 |
Study on Antitumor and Antioxidant Activities of the Endophytic Fungi Isolated from Artemisia annua |
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| QIAN Yi-xin,KANG Ji-chuan,GENG Kun,WANG Lu,LEI Bang-xing,HE.Study on Antitumor and Antioxidant Activities of the Endophytic Fungi Isolated from Artemisia annua[J].Journal of Fungal Research,2014(1):44-50. |
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| Authors: | QIAN Yi-xin KANG Ji-chuan GENG Kun WANG Lu LEI Bang-xing HE |
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| Institution: | Jun (1. Engineering and Research Center for Southwest Bio-pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang 550025, China; 2. College of Agronomy, Guizhou University, Guiyang 550025, China; 3.College of Pharrnacy, Guizhou University, Guiyang 550025, China; 4 Plant Protection and Quarantine Station of Guiyang City, Guiyang 550081, China) |
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| Abstract: | Antitumor and antioxidant activities of the ethyl acetate extract of 68 endophytic fungi associat- ed with Artemisia annua L. were evaluated by MTT method and DPPH radical scavenging method respec- tively. The results showed that 12 bioactive endophyfic fungi strains were obtained which were identified to belong to Alternaria, Colletotrichum, Pestalotiopsis and Phomopsis respectively by morphological char- acterisfics. Eight strains （11.8 % ） presented cytotoxic activities, at least to one of the test tumor cells, five strains （7.4 % ） exhibited scavenging effect of DPPH radical at different degrees and only one strain had eytotoxic and antioxidant activities simultaneously. Evidently, endophytic fungi in Artemisia annua are potential resources of valuable bioactive components, which should be researched more deeply. |
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| Keywords: | Artemisia annua L endophytic fungi cytotoxic activity antioxidant activity morphological identification |
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