The cloning and characterization of a RAS gene fromSchizosaccharomyces pombe |
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Authors: | Susan A Nadin-Davis Robert C A Yang Saran A Narang Anwar Nasim |
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Institution: | 1. Molecular Genetics Section, Division of Biological Sciences, National Research Council, 100 Sussex Drive, K1A 0R6, Ottawa, Ontario, Canada
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Abstract: | We have cloned and determined the complete nucleotide sequence of a RAS gene from the yeast Schizosaccharomyces pombe (SP-RAS). The putative RAS protein of 214 amino acids is encoded by two noncontiguous reading frames separated by an intron of 86 bp. The SP-RAS gene product shares extensive homology with the proteins of the Saccharomyces cerevisiae (SC), Dictyostelium, Drosophila, and human RAS genes in its N-terminal region but not in its C-terminal region. The extended C-terminal regions found in the SC-RAS genes have no counterpart in the SP-RAS gene. Thus the RAS genes of these two yeasts are structurally quite distinct. The SP-RAS sequence was expressed in vivo. |
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