| 混合Ribozyme联合切割小鼠腺苷脱氨酶mRNA研究 |
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| 引用本文： | 陈桦,陆长德.混合Ribozyme联合切割小鼠腺苷脱氨酶mRNA研究[J].生物化学与生物物理学报,1994,26(6):603-608. |
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| 作者姓名： | 陈桦 陆长德 |
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| 作者单位： | 中国科学院上海生物化学研究所 |
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| 基金项目： | 863和国家博士后科学基金 |
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| 摘 要： | 采用计算机辅助设计得到针对小鼠腺苷脱氨酶ｍＲＮＡ的４种ｒｉｂｏｚｙｍｅ，它们均能在各自的切点切割ＲＮＡ分子，４种ｒｉｂｏｚｙｍｅ经组合后成混合ｒｉｂｏｚｙｍｅ，它们分别含有２种、３种和４种ｒｉｂｏｚｙｍｓ。通过对体外转录靶ＲＮＡ分子的联合切割研究表明，随着ｒｉｂｏｚｙｍｅ种数的增加，其中以（Ｒｚ２６２＋Ｒｚ４５５＋Ｒｚ５８３）组成最为理想，由此证明混合ｒｉｂｏｚｙｍｅ中，各种ｒｉｂｏｚｙｍｅ不仅保
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| 关 键 词： | 联合切割 腺苷脱氨酶 mRNA 核酶 混合核酶 |
Co-cleavage of the Murine Adenosine Deaminase mRNA with Mixed Ribozymes |
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| CHEN Hua,LU Chang-De and QI Guo-Rong.Co-cleavage of the Murine Adenosine Deaminase mRNA with Mixed Ribozymes[J].Acta Biochimica et Biophysica Sinica,1994,26(6):603-608. |
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| Authors: | CHEN Hua LU Chang-De and QI Guo-Rong |
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| Abstract: | With computer design of the adenosine deaminase(ADA)mRNA specific ribozymes,4 ribozymes directed to nucleotide 262,455,583 and 862 on ADA mRNA were selected.They can specifically cleave the ADA mRNA at their respective site. These ribozymes were combined into mixed ribozymes.With increasing kinds of ribozymes,the cleavage products increased and the cleavage rate elevated.Among the mixed ribozymes,the combination of Rz262+Rz455+ Rz583 was the most effective one.These results demonstrated that the mixed ribozymes not only can retain their specificity,but also cooperate to increase the cleavage efficiency in blocking the target gene expression. |
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| Keywords: | Mixed ribozymes Co-cleavage Adenosine deaminase inRNA |
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