| 产植酸酶菌株的筛选及其植酸酶基因的克隆 |
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| 引用本文： | 王严,高晓蓉,苏乔,王静云,安利佳.产植酸酶菌株的筛选及其植酸酶基因的克隆[J].微生物学通报,2007,34(4):0719-0722. |
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| 作者姓名： | 王严 高晓蓉 苏乔 王静云 安利佳 |
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| 作者单位： | 大连理工大学环境与生命科学学院生物工程系,大连,116024 |
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| 摘 要： | 从土壤中筛选到产植酸酶活性较高的烟曲霉菌株WY-2,其植酸酶最适pH为5.5,最适温度为55℃。通过对烟曲霉WY-2植酸酶基因进行PCR扩增,获得了一个1.5kb大小的特异性产物,将其克隆到载体pMD18-T中。测序结果分析表明,该基因片段含有植酸酶基因完整的阅读框架(ORF),基因全长1459bp,其中包含一个61bp的内含子,编码465个氨基酸,有7个潜在的糖基化位点,5′端有一编码26个氨基酸的信号肽序列。该基因与已报道的烟曲霉ATCC34625植酸酶基因有91%同源性,编码的氨基酸序列同源性为91%。
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| 关 键 词： | 烟曲霉 植酸酶基因 克隆 |
| 文章编号： | 0253-2654（2007）04-0719-04 |
| 修稿时间： | 2006-11-29 |
Screening of Phytase-producing Strain and Cloning of Phytase Gene from the Selected Strain |
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| WANG Yan,GAO Xiao-Rong,SU Qiao,WANG Jing-Yun and AN Li-Jia.Screening of Phytase-producing Strain and Cloning of Phytase Gene from the Selected Strain[J].Microbiology,2007,34(4):0719-0722. |
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| Authors: | WANG Yan GAO Xiao-Rong SU Qiao WANG Jing-Yun and AN Li-Jia |
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| Institution: | Department of Bioscience and Biotechnology, School of Environment and Life, Dalian University of Technology, Dalian 116024;Department of Bioscience and Biotechnology, School of Environment and Life, Dalian University of Technology, Dalian 116024;Department of Bioscience and Biotechnology, School of Environment and Life, Dalian University of Technology, Dalian 116024;Department of Bioscience and Biotechnology, School of Environment and Life, Dalian University of Technology, Dalian 116024;Department of Bioscience and Biotechnology, School of Environment and Life, Dalian University of Technology, Dalian 116024 |
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| Abstract: | |
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| Keywords: | Aspergillus fumigatus Phytase gene Cloning |
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