A Method for Isolation of Chloroplast DNA and Mitochondrial DNA from Sunflower |
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| Authors: | S.O. Triboush N.G. Danilenko O.G. Davydenko |
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| Affiliation: | (1) Department of Extranuclear Heredity, National Academy of Sciences, Institute of Genetics & Cytology, 27 Academicheskaja str., 220072 Minsk, Belarus |
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| Abstract: | ![]()
We present a method for isolation of chloroplast and mitochondrial DNA from sunflower seedlings. The protocol includes: organelle isolation, deoxyribonuclease treatment, lysis, deproteinisation and a final DNA purification with sodium dodecyl sulphate and potassium acetate. The organelle DNA yield is 5–10 micrograms per gram of tissue and the DNA is fully restrictable. The technique is inexpensive and appropriate for the isolation of multiple samples of organelle DNA from a small amount of tissue. |
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| Keywords: | chloroplast DNA mitochondrial DNA organelle isolation sunflower |
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