| 两种藻胆蛋白对人肝癌SMMC-7721细胞的光动力体外杀伤效应 |
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| 引用本文： | 王源,蔡春尔,李柏林,刘承初,何培民.两种藻胆蛋白对人肝癌SMMC-7721细胞的光动力体外杀伤效应[J].生物工程学报,2009,25(9):1417-1423. |
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| 作者姓名： | 王源 蔡春尔 李柏林 刘承初 何培民 |
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| 作者单位： | 上海海洋大学,上海,201306 |
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| 基金项目： | 国家高技术研究发展计划(863计划)(Nos.2006AA10Z323,2007AA09Z406);;教育部水产种质资源发掘与利用省部共建重点开放实验室(No.KFT2008-12);;上海市教委优势(重点)学科项目(No.S30701)资助~~ |
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| 摘 要： | 用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法测定提取自条斑紫菜的R-藻红蛋白(R-PE)和C-藻蓝蛋白(C-PC)经激光介导对人肝癌SMMC-7721细胞的杀伤率,并检测细胞凋亡率。结果显示在120mg/L浓度下,R-PE在氩离子激光器100J/cm2辐照下对应的细胞存活率为27%,C-PC在He-Ne激光器35J/cm2辐照下对应的细胞存活率为47%;单用2种激光器辐照对应的细胞存活率分别为65%和70%;单用2种蛋白处理细胞在72h后出现对细胞生长的显著抑制,最大抑制率分别为31%(R-PE,120mg/L)和27%(C-PC,250mg/L)。120mg/L的2种藻胆蛋白在对应激光辐照下,于照后8h达到细胞凋亡率最大值,分别为31.54%(R-PE)和32.54%(C-PC)。本实验证明条斑紫菜R-PE和C-PC具有可开发成为光敏剂的应用前景。
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| 关 键 词： | 条斑紫菜 R-藻红蛋白 C-藻蓝蛋白 光敏作用 细胞凋亡 肝癌细胞 |
| 收稿时间： | 2009/4/22 0:00:00 |
Photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line SMMC-7721 in vitro |
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| Yuan Wang,Chuner Cai,Bailin Li,Chengchu Liu and Peimin He.Photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line SMMC-7721 in vitro[J].Chinese Journal of Biotechnology,2009,25(9):1417-1423. |
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| Authors: | Yuan Wang Chuner Cai Bailin Li Chengchu Liu and Peimin He |
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| Institution: | Shanghai Ocean University, Shanghai 201306, China;Shanghai Ocean University, Shanghai 201306, China;Shanghai Ocean University, Shanghai 201306, China;Shanghai Ocean University, Shanghai 201306, China;Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | We studied the effect of photodynamic therapy with phycobiliproteins on human liver cancer cells in vitro.With 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay(MTT assay),we used two phycobiliproteins,R-phycoerythrin(R-PE) and C-phycocyanin(C-PC) prepared from Porphyra yezoensis,to determine the killing rates and apoptosis rates of human liver cancer cells(SMMC-7721) mediated by laser.When the concentration of R-PE was 120 mg/L,the survival rate of human liver cancer cells was 27% after tr... |
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| Keywords: | Porphyra yezoensis R-phycoerythrin C-phycocyanin photodynamic therapy cell apoptosis liver cancer cell |
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