Effect of container,vitrification volume and warming solution on cryosurvival of in vitro-produced bovine embryos |
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| Authors: | G.L. Rios N.C. MucciG.G. Kaiser R.H. Alberio |
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| Affiliation: | Laboratorio de Producción in vitro de Embriones, Biotecnología de la Reproducción, Departamento de Producción Animal, INTA, CC 276 (7620) Balcarce, Buenos Aires, Argentina |
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| Abstract: | The aim of the present research was to develop a low cost and easy to perform vitrification method for in vitro-produced cattle embryos. Effect of container material was evaluated (plastic straw compared to glass capillary, experiment 1), two volume sample (1 compared to 0.5 μL, experiment 2) and warming solution composition medium (Tissue Culture Medium 199 (TCM-199) compared to phosphate buffered saline (PBS), experiment 3) as modifications of the open pulled straw (OPS) system in order to reduce embryo damage caused by exposure to cold. |
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| Keywords: | Cattle Vitrification Glass capillary OPS Embryo In vitro |
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