Sucrose hydrolysis using invertase immobilized on concanavalin A-sepharose |
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Authors: | Jawaid Iqbal M. Saleemuddin |
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Affiliation: | Biochemistry Division, Department of Chemistry, Aligarh Muslim University, Aligarh 202001, India |
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Abstract: | The stability and ability of yeast invertase (β-d-fructofuranoside fructohydrolase, EC 3.2.1.26) bound and covalently coupled to concanavalin A-Sepharose continuously to hydrolyse sucrose over long periods has been investigated. The immobilized preparation exhibited high resistance to heat and urea induced denaturation. A small column of the immobilized preparation could hydrolyse relatively high concentrations of sucrose almost quantitatively for more than 60 days. |
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Keywords: | Enzyme glycoenzyme immobilization crosslinking enzyme stabilization sucrose inversion |
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