| 固氮酶铁钼辅因子FeMo-co在水与非水体系内重组活力与催化活力的比较研究 |
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| 引用本文： | 罗贵民,C.E.McKenna.固氮酶铁钼辅因子FeMo-co在水与非水体系内重组活力与催化活力的比较研究[J].中国生物化学与分子生物学报,1990,6(5):390-393. |
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| 作者姓名： | 罗贵民 C.E.McKenna |
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| 作者单位： | 吉林大学酶工程实验室 长春
(罗贵民)，南加利福尼亚大学化学系 美国(C.E.McKenna) |
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| 摘 要： | 本文提出一种测定FeMo-co催化活力的反应体系,用此反应体系,在测定FeMo-co催化活力的过程中,FeMo-co与变种UW45抽提液的重组活性始终保持不变。讨论了水含量、还原剂对FeMo-co催化活力和重组活力的影响。
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| 关 键 词： | 固氮酶铁钼辅因子 固氮酶 催化活性测定 重组活性分析 |
| 收稿时间： | 1990-10-20 |
Comparision between Reconstitutive Activity and Catalytic Activity of FeMo cofactors in water and Water-free Reaction Systems |
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| Luo,Gui-Min C.E. McKenna.Comparision between Reconstitutive Activity and Catalytic Activity of FeMo cofactors in water and Water-free Reaction Systems[J].Chinese Journal of Biochemistry and Molecular Biology,1990,6(5):390-393. |
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| Authors: | Luo Gui-Min CE McKenna |
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| Institution: | (Lab of Enzyme Engineering, Jilin University, Changchun Department of Chemistry, University of Southern California, U.S.A. |
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| Abstract: | A water-free reaction system for determining catalytic activity of Fe-Mo-co has been developed. Comparison between reconstitutive activity and catalytic activity of FeMo-co in water and water-free systems has been made. The results show that free FeMo-co requires hydrophilic environment for its catalytic activity, while the FeMo-co in reconstituted form requires hydrophobic environment for its reconstitution activity. The effects of water content and reductant concentration in the reaction systems on catalytic activity of FeMo-co were discussed. |
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| Keywords: | FeMo cofactor Nitrogenase Reconstitutive activity assay Catalytic activity determination |
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