实验动物运输盒微生物指标检测分析

目的检测SPF金黄地鼠在从204车间转运到隔离器车间时微生物污染情况,同时对不同运输盒进行比较,筛选合格的实验动物运输盒。方法用TSA培养皿模拟实验动物运输过程,比较沉降菌检测结果。结果大小IVC笼盒内环境微生物能控制在14 cfu/4 h以下,而且菌落形态与SPF饲养间环境内的沉降菌基本一致,符合标准要求;甲乙普通运输盒内环境微生物却在70 cfu/4 h以上,不符合标准要求。结论 SPF金黄地鼠在从204车间转运到隔离器车间时未受到污染;实验动物转移运输应选择大小IVC笼盒。

Comparison of Microbial Pollution during Transfer of Laboratory Animals between Production Workshops Using Different Transport Boxes

Objective To determine whether the SPF hamsters transfered from building 204 to isolator workshop were microbially polluted using different trasport boxes,and select qualified boxes for laboratory animal transportation.Methods Using triptic soy agar（TSA） culture dishes,simulating laboratory animal transportation process,and compare the results of settling plates in different boxes.Results The microorganisms in individually ventilated cages cages（IVC） could be controlled bellow 14 cfu/4 h,and the morphology was the same with the colony in SPF building,therefore,were accord with standard.But in the conventional transport boxes,the colony were above 70 cfu/4 h,which failed to meet the standards.Conclusions SPF hamsters are not microbially polluted during the transport from building 204 to the isolator workshop using IVC cages.IVC cages are much better than conventional trasport boxes and should be used in laboratory animal transportation.