GF级KM小鼠生长曲线、主要脏器参数及生理生化指标的测定分析

目的采用对SPF级KM小鼠进行剖宫产术,用无菌奶妈代乳方法,培育出GF级（即无菌级）KM小鼠,了解GF级KM小鼠的生长发育并绘制出生长曲线;测定其不同生长阶段的主要脏器重量及血液生理生化值并进行比较分析。方法①分别称取60只（雌雄各半）0～112日龄GF级KM小鼠体重,绘制其生长曲线;②随机抽取28、56、112日龄的KM小鼠60只（雌雄各半）,活体称重,依次剖取心、肝等主要脏器称重;③小鼠眼眶采血,测定其血常规和生化值。结果 GF级KM小鼠的体重随着日龄的增长而逐渐增加,断奶后1～2周内增重最为迅速,4周后的雄鼠体重明显高于雌鼠;主要脏器（心、肝、脾、肺、脑等）重量随年龄增大均逐渐增加,盲肠体积增大尤其明显,而胸腺随着日龄的增加而逐渐萎缩,其重量迅速减轻;同一日龄GF级KM小鼠的雌雄间血常规和生化值指标均存在差异,但随着年龄的增加差异的项目逐渐增多。结论 GF级KM小鼠生长发育和血液生理生化的研究结果可为KM小鼠的标准化及最终成为国际标准品系提供基础数据。

Analysis of Growth Curve,Organ Parameter and Physiology and Biochemistry Indicators in Germ-free KM Mice

Objective To determine the growth rate,organ index,blood Physiological and biochemical Parameters of germ-free KM mice during different growing Period,baby germ-free KM mice were obtained by hysterectomy form SPF KM mice and reared by hand feeding under aseptic condition.Methods（1） Weight and body size of the mice were measured at different days（from 0 to 112days;n=60;30♀,30♂） to create a growth curve（2） The weight of the live mice and major organ size after dissection were measured at different days selected randomly（day = 28,56,112;n = 60 30♀,30♂） to determine the growth of these mice.（3） The blood Physiological and biochemical Parameters were measured by collected blood samples from the infraorbital vessels.Results（1） The weight of germ-free KM mice increased with the growing days,and the growth rate reached maximum within 1-2weeks Post-weaning.Significant difference was found between males and females（P0.05） after 4th week.（2） All the major organs（heart,liver,spleen,lung and brain） increased with the ages except the thyme gland,and the growth of cecum size was obvious.（3） Difference of blood Physiological and biochemical Parameters was found between males and females at the same age,and the difference types increased with the growth of mice age.Conclusion The results of growth rate and blood physiological and biochemical parameters could provide reference for the evaluation of germ-free KM mice colony.