Performance of a horizontal-flow anaerobic immobilized biomass (HAIB) reactor and dynamics of the microbial community during degradation of pentachlorophenol (PCP) |
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| Authors: | Baraldi Elizabeth A Damianovic Márcia H R Z Manfio Gilson P Foresti Eugenio Vazoller Rosana F |
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| Institution: | 1. Laboratório de Processos Biológicos, Escola de Engenharia de São Carlos, Universidade de São Paulo, São Carlos - SP, Brazil;2. Gerência de Tecnologia Química da Natura Inovação e Tecnologia de Produtos Ltda., Cajamar - SP, Brazil;3. Laboratório de Microbiologia Ambiental, Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo - SP, Brazil;1. Departamento de Engenharia Hidráulica e Ambiental (PHD), Escola Politécnica (EP), Universidade de São Paulo (USP), Avenida Prof. Almeida Prado, 83 trav. 2, Cidade Universitária, CEP: 05508-900, São Paulo, SP, Brazil;2. HagaPlan Engenharia e Serviços, Praça Ramos de Azevedo, 206 – 26° andar, Conjuntos 2610/2620 – Edifício Esplanada – República, CEP: 01037-010, São Paulo, SP, Brazil;3. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Av. 24-A, 1515, Bela vista, CEP: 13506-900, Rio Claro, SP, Brazil;4. Laboratório de Processos Biológicos (LPB), Centro de Pesquisa Desenvolvimento e Inovação em Engenharia Ambiental, Departamento de Hidráulica e Saneamento (SHS), Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental, Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, CEP: 13.563-120, São Carlos, SP, Brazil;5. Departamento de Hidráulica e Saneamento (SHS), Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Avenida Trabalhador São-carlense, 400, Centro, CEP: 13566-590, São Carlos, SP, Brazil;1. Department of Geography, Queen’s University, Mackintosh-Corry Hall, Room D201, Kingston, ON K7L 3N6, Canada;2. Department of Physics and Astronomy, 834 Campus Place N.W, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada;3. BSc Environmental Science Program, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada |
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| Abstract: | The anaerobic biological treatment of pentachlorophenol (PCP) and methanol as the main carbon source was investigated in a horizontal-flow anaerobic immobilized biomass (HAIB) reactor at 30+/-1 degrees C, during a 220-day trial period. The reactor biomass was developed as an attached biofilm on polyurethane foam particles, with 24h of hydraulic retention time. The PCP concentrations, which ranged from 2.0 to 13.0 mg/L, were controlled by adding synthetic substrate. The HAIB reactor reduced 97% of COD and removed 99% of PCP. The microbial biofilm communities of the HAIB reactor amended with PCP, without previous acclimatization, were characterized by polymerase chain reaction (PCR) and amplified ribosomal DNA restriction analysis (ARDRA) with specific Archaea oligonucleotide primers. The ARDRA technique provided an adequate analysis of the community, revealing the profile of the selected population along the reactor. The biomass activities in the HAIB reactor at the end of the experiments indicated the development of PCP degraders and the maintenance of the population of methanogenic Archaea, ensuring the high efficiency of the system treating PCP with added methanol as the cosubstrate. The use of the simplified ARDRA method enabled us to monitor the microbial population with the addition of high concentrations of toxic compounds and highlighting a selection of microorganisms in the biofilm. |
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