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| 四环素调控A30P突变α-synuclein转基因小鼠的构建 | |
| 引用本文： | 胡伟东,杨亚萍,程言博,钱进军,李莉,张萍,石际俊,刘春风.四环素调控A30P突变α-synuclein转基因小鼠的构建[J].动物学杂志,2009,44(2):64-70. |
| 作者姓名： | 胡伟东 杨亚萍 程言博 钱进军 李莉 张萍 石际俊 刘春风 |
| 作者单位： | 1. 苏州大学附属第二医院神经内科,苏州,215004 2. 苏州大学附属第二医院神经内科,苏州,215004;苏州大学衰老与神经疾病实验室,苏州,215123 3. 扬州大学比较医学中心,扬州,225009 |
| 基金项目： | 江苏省脑病生物信息重点实验室开放课题，苏州大学高校省级重点实验室开放课题 |
| 摘 要： | 为了构建四环素调控的人A30P突变α-synuclein转基因小鼠模型,将外源基因pTRE2-syn和pBC-rtTA同时显微注射到FVB小鼠(Mus muscculus)受精卵的雄原核中,将注射后存活的受精卵移植到同期发情的假孕受体鼠输卵管中,出生个体经PCR检测,获得rtTA和A30P突变α-synuclein双阳性转基因雌鼠1只,A30P单基因阳性雄鼠13只并传代.强力霉索诱导后双阳性后代脑区各部分A30P突变α-synucleinmRNA均有表达,而在诱导满4周后,脑干α-synuclein蛋白表达明显增加,8周后增加更明显.结果表明,通过强力霉素诱导后,可在小鼠小脑、脑干、海马、皮层检测到A30P mRNA表达,脑干α-synuclein表达量显著增加. |
| 关 键 词： | 四环素 α-synuclein 转基因 构建 |
| 收稿时间： | 9/9/2008 12:00:00 AM |
| 修稿时间： | 2008/12/30 0:00:00 |
Establishment of Tetracycline-controlled A30P Mutation α-synuclein Transgenic Mice |
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| HU Wei-Dong,YANG Ya-Ping,CHENG Yan-Bo,QIAN Jin-Jun,LI Li,ZHANG Ping,SHI Ji-Jun and LIU Chun-Feng.Establishment of Tetracycline-controlled A30P Mutation α-synuclein Transgenic Mice[J].Chinese Journal of Zoology,2009,44(2):64-70. | |
| Authors: | HU Wei-Dong YANG Ya-Ping CHENG Yan-Bo QIAN Jin-Jun LI Li ZHANG Ping SHI Ji-Jun and LIU Chun-Feng |
| Institution: | Department of Neurology,The Second Affiliated Hospital of Suzhou University,Suzhou 215004;Department of Neurology,The Second Affiliated Hospital of Suzhou University,Suzhou 215004; Laboratory of Aging and Nervous Diseases,Suzhou University,Suzhou 215123;Department of Neurology,The Second Affiliated Hospital of Suzhou University,Suzhou 215004;Department of Neurology,The Second Affiliated Hospital of Suzhou University,Suzhou 215004;The Comparative Medicine Center of Yangzhou University,Yangzhou 225009,China;Department of Neurology,The Second Affiliated Hospital of Suzhou University,Suzhou 215004;Department of Neurology,The Second Affiliated Hospital of Suzhou University,Suzhou 215004;Department of Neurology,The Second Affiliated Hospital of Suzhou University,Suzhou 215004; Laboratory of Aging and Nervous Diseases,Suzhou University,Suzhou 215123 |
| Abstract: | |
| Keywords: | A30P |
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