Aldosterone plasma radioimmunoassay interference by a spirolactone metabolite. |
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| Authors: | W Sadée A M Finn P Schmiedek A Baethmann |
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| Affiliation: | 1. Schools of Pharmacy, University of California, San Francisco and University of Southern California, Los Angeles, California, U.S.A.;2. Institut für Chirurgische Forschung, Chirurgische Klinik der Universitaet München, Germany |
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| Abstract: | The plasma aldosterone radioimmunoassay developed by Ito et al. was found to be non-specific for aldosterone following administration of the spirolactones, spironolactone and canrenoate-K, in rabbits, dogs and humans. The assay interfering principle was identified as a hydroxylated derivative (M-B) of canrenone, which itself is a metabolite common to both spironolactone and canrenoate-K. The metabolite M-B possessed a high cross-reactivity to the 21-hemisuccinate aldosterone antibody relative to other spirolactones. A modified procedure was developed specific for plasma aldosterone in the presence of M-B. Following single doses of spironolactone and canrenoate-K, aldosterone plasma levels were unchanged in humans and in dogs and decreased in rabbits. |
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| Keywords: | Reprint requests to: Dr. W. Sadée School of Pharmacy University of California San Francisco California 94143. |
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