The action of lipase on chloroplast membranes: II. Polypeptide patterns of bean galactolipase- and phospholipase A2-treated thylakoid membranes |
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Authors: | Z Krupa |
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Institution: | (1) Institute of Biology, Maria Curie-Skiodowska University, Lublin, Poland |
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Abstract: | Thylakoid membranes obtained from bean chloroplasts treated with bean galactolipase or phospholipase A2 (from Crotalus terr. terr.) showed marked changes in their polypeptide patterns when separated on SDS-PAGE. The obtained results have been discussed with regard to the relationship between chloroplast lipids and polypeptides originating from chlorophyll-protein complexes of bean thylakoids. A coexistence between galactolipids and the peripheral antennae in PS I complex and LHCP3 as well as a conspicuous role of phospholipids in PSI and PSII centre chlorophyll-protein complexes has to be underlined.Abbreviations CP1
chlorophyll a-protein complex of PSI
- CPa
chlorophyll a-protein complex of PSII
- D10
digitonin subchloroplast particles enriched in PSII
- D144
digitonin subchloroplast particles enriched in PSI
- DCMU
3-(3,4-dichlorophenyl)-1, 1-dimethylurea
- LHCP1-3
light harvesting chlorophyll a/b protein complexes
- PAGE
polyacrylamide gel electrophoresis
- PSI
photosystem I
- PSII
photosystem II
- SDS
sodium dodecyl sulphate
- TCA
trichloroacetic acid
- Tricine
N-Tris-(hydroxymethyl)-methylglycine
- Tris
Tris-(hydroxymethyl)-aminomethan |
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Keywords: | chloroplast lipids gel electrophoresis polypeptides thylakoid membranes treatment with bean galactolipase treatment with phospholipase A2 |
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