UAE-IC法测定大白口蘑中阴离子含量

采用超声波（UAE）辅助提取-离子色谱（IC）法测定了大白口蘑中F?、Cl?、NO2?、NO3?、PO43? 5种阴离子。选用NaOH溶液（3.0mmol/L）超声渗提30min后进样100μL，通过流速为1.2mL/min的淋洗液Na2CO3+NaHCO3（3.5mmol/L+1.0mmol/L）洗脱。F?、Cl?、NO2?、NO3?、PO43? 5种离子线性范围分别为2－25mg/L、2－20mg/L、2－100mg/L、10－55mg/L、10－100mg/L，峰面积RSD分别为0.58%、1.27%、0.73%、0.92%、2.33%，检出限在0.0976－1.0984mg/L之间。大白口蘑样品中未检出NO2?，其余F?、Cl?、NO3?、PO43? 4种离子加标回收率在95%－110%之间。

Determination of anions in Tricholoma giganteum fruiting body by ultrasonic assisted extraction-ion chromatography

Five anions (F-,Cl-,NO2-,PO43-,NO3-) in Tricholoma giganteum fruiting body were determined by using ultrasonic assisted extraction (UAE)-ion chromatography (IC).After ultrasonic assisted extraction for 30min in NaOH solvent (3.0mmol/L),sample introduction as 100μL,Na2CO3+NaHCO3 (3.5mmol/L+1.0mmol/L) were chosen as washing solution with flowing rate at 1.2mL/min.The linearity ranges of 5 anions respectively were 2-25mg/L,2-20mg/L,2-100mg/L,10-55mg/L,10-100mg/L.The relative standard deviations (RSD) of the peak areas were 0.58%,1.27%,0.73%,0.92%,2.33%,respectively.The detection limits of the 5 anions were 0.0976-1.0984mg/L.The spike recoveries of 4 anions (F-,Cl-,PO43-,NO3-) were 95%-110%,whereas,the content of NO2- in T.giganteum was not detected out.