Laboratory production and characterization of a new biosurfactant from <Emphasis Type="Italic">Candida glabrata</Emphasis> UCP1002 cultivated in vegetable fat waste applied to the removal of hydrophobic contaminant |
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Authors: | Carolina A B de Gusmão Raquel D Rufino Leonie A Sarubbo |
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Institution: | 1.Catholic University of Pernambuco,Recife,Brazil;2.Federal University of Pernambuco,Recife,Brazil;3.Center of Science and Technology,Catholic University of Pernambuco,Recife,Brazil |
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Abstract: | Biosurfactant production by Candida glabrata was studied using vegetable fat waste as substrate. A factorial design was initially carried out to investigate the effects
and interactions of waste, yeast extract and glucose on the surface tension after 144 h cultivation. Maximum surface tension
reduction was achieved with vegetable fat waste at 5% and yeast extract at 0.2%. The biosurfactant containing cell-free broth
retained its surface-active properties after incubation at high temperatures, at a wide range of pH values and salt concentrations.
Comparison between three solvent systems for surfactant recovery showed that ethyl acetate extracted both crude extracellular
and intracellular biosurfactant with high product recovery. The isolated extracellular biosurfactant showed a CMC of 1% and
the surface tension at that point was 24 mN m−1. Preliminary chemical composition revealed the presence of carbohydrates, proteins and lipids. The application of the crude
biosurfactant to a soil–water-hydrophobic contaminant system was investigated and the apparent critical micelle concentration
was determined at 7% of the broth, although the best oil removal (92.6%) had been obtained with 10% of the cell-free broth.
The cost of application of the biosurfactant in soils was estimated based on the cost of a commercial biosurfactant. |
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