Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase |
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Authors: | Chen Mo Lichtler Alexander C Sheu Tzong-Jen Xie Chao Zhang Xinping O'Keefe Regis J Chen Di |
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Institution: | Department of Orthopaedics, Center for Musculoskeletal Research, University of Rochester School of Medicine, Rochester, New York 14642, USA. |
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Abstract: | Postnatal cartilage development and growth are regulated by key growth factors and signaling molecules. To fully understand the function of these regulators, an inducible and chondrocyte-specific gene deletion system needs to be established to circumvent the perinatal lethality. In this report, we have generated a transgenic mouse model (Col2a1-CreER(T2)) in which expression of the Cre recombinase is driven by the chondrocyte-specific col2a1 promoter in a tamoxifen-inducible manner. To determine the specificity and efficiency of the Cre recombination, we have bred Col2a1-CreER(T2) mice with Rosa26R reporter mice. The X-Gal staining showed that the Cre recombination is specifically achieved in cartilage tissues with tamoxifen-induction. In vitro experiments of chondrocyte cell culture also demonstrate the 4-hydroxy tamoxifen-induced Cre recombination. These results demonstrate that Col2a1-CreER(T2) transgenic mice can be used as a valuable tool for an inducible and chondrocyte-specific gene deletion approach. |
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Keywords: | chondrocyte Cre‐mediated recombination conditional knockout tamoxifen X‐Gal staining |
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