WDNM1 is Associated with Differentiation and Apoptosis of Mammary Epithelial Cells |
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| Authors: | Yoonjung Kho∗ Sungchan Kim∗ Byung Sun Yoon∗ Jai-Hee Moon Sungwook Kwak Gyuman Park |
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| Institution: | 1. School of Agricultural and Biotechnology , College of Agriculture and Life Sciences Seoul National University , Seoul, South Korea;2. Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology , Korea University , Seoul, South Korea;3. Department of Biochemistry , College of Medicine, Hallym University , Chuncheon, South Korea;4. Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology , Korea University , Seoul, South Korea;5. School of Agricultural and Biotechnology , College of Agriculture and Life Sciences Seoul National University , Seoul, South Korea |
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| Abstract: | In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFα treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFα induced WDNM1 expression showed the NFκB-dependent mechanism since it's expression was abrogated in IκBαM (super-repressor of NFκB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNFα-NFκB signal pathway, is associated with HC11 cell apoptosis. |
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| Keywords: | Apoptosis Mammary epithelial cell κB" target="_blank">NFκB α" target="_blank">TNFα WDNM1 |
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