Purification and characterization of rhodobactin: a mixed ligand siderophore from Rhodococcus rhodochrous strain OFS |
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| Authors: | Suraj Dhungana Ryszard Michalczyk Hakim Boukhalfa Joseph G Lack Andrew T Koppisch Jason M Fairlee Mitchell T Johnson Christy E Ruggiero Seth G John Matthew M Cox Cindy C Browder Jennifer H Forsythe Laura A Vanderberg Mary P Neu Larry E Hersman |
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| Institution: | (1) Bioscience, Los Alamos National Laboratory, Mail Stop M888, Los Alamos, NM 87545, USA;(2) Chemistry Divisions, Los Alamos National Laboratory, Mail stop J514, Los Alamos, NM 87545, USA;(3) Department of Chemistry, Fort Lewis College, 1000 Rim Dr., Durango, CO 81301, USA;(4) Present address: National Institute of Environmental Health Sciences (NIEHS), 111 T W Alexander Drive, RTP, NC 27709, USA |
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| Abstract: | The siderophore produced by Rhodococcus rhodochrous strain OFS, rhodobactin, was isolated from iron-deficient cultures and purified by a combination of XAD-7 absorptive/partition
resin column and semi-preparative HPLC. The siderophore structure was characterized using 1D and 2D 1H, 13C and 15N NMR techniques (DQFCOSY, TOCSY, NOESY, HSQC and LR-HSQC) and was confirmed using ESI-MS and MS/MS experiments. The structural
characterization revealed that the siderophore, rhodobactin, is a mixed ligand hexadentate siderophore with two catecholate
and one hydroxamate moieties for iron chelation. We further investigated the effects of Fe concentrations on siderophore production
and found that Fe limiting conditions (Fe concentrations from 0.1 μM to 2.0 μM) facilitated siderophore excretion. Our interests
lie in the role that siderophores may have in binding metals at mixed contamination sites (containing metals/radionuclides
and organics). Given the broad metabolic capacity of this microbe and its Fe scavenging ability, R. rhodochrous OFS may have a competitive advantage over other organisms employed in bioremediation.
Electronic Supplementary Material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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| Keywords: | Rhodococcus Rhodobactin Siderophore Catecholate Hydroxamate |
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