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Linc00475 promotes the progression of glioma by regulating the miR-141-3p/YAP1 axis |
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| Authors: | Mingjun Yu Bolong Yi Wen Zhou Wei Gong Gang Li Shijia Yu |
| Institution: | 1. Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China
Gamma Knife Center, Shengjing Hospital of China Medical University, Shenyang, China Key Laboratory of Neuro-oncology in Liaoning Province, Shenyang, China Contribution: Data curation (equal), Funding acquisition (equal), ?Investigation (lead), Writing - original draft (lead), Writing - review & editing (equal);2. Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China Key Laboratory of Neuro-oncology in Liaoning Province, Shenyang, China Contribution: Formal analysis (equal), Methodology (lead), Writing - review & editing (equal);3. Department of Pain Management, Dalian Municipal Central Hospital, Dalian, China Contribution: Formal analysis (equal), Software (equal), Writing - review & editing (equal);4. Exprimental Research Center, Shengjing Hospital of China Medical University, Shenyang, China Contribution: Data curation (supporting), ?Investigation (equal), Methodology (equal);5. Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China Contribution: Formal analysis (equal), Methodology (equal), Software (equal);6. Department of Neurology, Shengjing Hospital of China Medical University, Shenyang, China |
| Abstract: | Glioma is the most prevalent and lethal primary brain tumour. Abundant long non-coding RNAs ( lncRNAs) are aberrant and play crucial roles in the oncogenesis of glioma. The exact functions of linc00475 in glioma remain blurred. Here, we analysed the expression levels of linc00475 by qRT-PCR and discovered that linc00475 was up-regulated in glioma and predicted a poor prognosis in patients with glioma. Besides, inhibiting linc00475 restrained the progression of glioma in vitro and in vivo. Further experiments confirmed that linc00475 regulated the progression of glioma by acting as a sponge for miR-141-3p. Moreover, we detected the binding sites of linc00475 and miR-141-3p, the YAP1- 3′UTR and miR-141-3p by luciferase reporters. The rescue assays confirmed that inhibiting linc00475 restrained the progression of glioma through the miR-141-3p/YAP1 pathway. Collectively, our research demonstrates the key roles of linc00475 in glioma, which could be a promising therapeutic target. |
| Keywords: | ceRNA glioma linc00475 miR-141-3p prognosis YAP1 |