|
|||||
|
|
Expression profiles and potential roles of transfer RNA-derived small RNAs in atherosclerosis |
|
| Authors: | Xiangqin He Yanyan Yang Qi Wang Jueru Wang Shifang Li Chunrong Li Tingyu Zong Xiaolu Li Ying Zhang Yulin Zou Tao Yu |
| Institution: | 1. Department of Cardiac Ultrasound, The Affiliated Hospital of Qingdao University, Qingdao, China;2. Department of Immunology, Basic Medicine School, Qingdao University, Qingdao, China
Contribution: Conceptualization (equal), Funding acquisition (equal), Methodology (equal), Resources (equal), Software (equal), Writing - review & editing (equal);3. Department of Cardiology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi'an, Shaanxi, China Contribution: Formal analysis (equal), Methodology (equal), Software (equal), Validation (equal), Visualization (equal), Writing - original draft (equal);4. The department of thyroid surgery, The Affiliated Hospital of Qingdao University, Qingdao, China Contribution: Resources (supporting);5. Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, China Contribution: Resources (supporting);6. Department of Cardiac Ultrasound, The Affiliated Hospital of Qingdao University, Qingdao, China Contribution: Investigation (supporting), Methodology (supporting), Resources (supporting) |
| Abstract: | Knowledge regarding the relationship between the molecular mechanisms underlying atherosclerosis (AS) and transfer RNA-derived small RNAs (tsRNAs) is limited. This study illustrated the expression profile of tsRNAs, thus exploring its roles in AS pathogenesis. Small RNA sequencing was performed with four atherosclerotic arterial and four healthy subject samples. Using bioinformatics, the protein-protein interaction network and cellular experiments were constructed to predict the enriched signalling pathways and regulatory roles of tsRNAs in AS. Of the total 315 tsRNAs identified to be dysregulated in the AS group, 131 and 184 were up-regulated and down-regulated, respectively. Interestingly, the pathway of the differentiated expression of tsRNAs in cell adhesion molecules (CAMs) was implicated to be closely associated with AS. Particularly, tRF-Gly-GCC might participate in AS pathogenesis via regulating cell adhesion, proliferation, migration and phenotypic transformation in HUVECs and VSMCs. In conclusion, tsRNAs might help understand the molecular mechanisms of AS better. tRF-Gly-GCC may be a promising target for suppressing abnormal vessels functions, suggesting a novel strategy for preventing the progression of atherosclerosis. |
| Keywords: | atherosclerosis cell proliferation expression profile non-coding RNAs tRNA-derived fragments |