miR-19a 在正常妊娠及重度子痫前期患者胎盘中的表达

目的:通过检测正常妊娠及重度子痫前期患者胎盘组织中miR-19a的表达,探讨其与子痫前期发病的关系。方法:收集10例重度子痫前期患者胎盘组织(实验组)和10例正常产妇胎盘组织(对照组),应用荧光实时定量PCR(Real Time PCR)的方法检测两组miR-19a的表达差异。结果:重度子痫前期患者胎盘组织中miR-19a表达升高(P<0.05)。结论:子痫前期患者胎盘组织中存在差异表达的miRNA,miR-19a在胎盘组织中的高表达可能与子痫前期的发病有关。

Expression of MiR-19a in Placentas From Women with Preeclampsia and Normal Pregnancy

Objective: To study the relationship of the pathogenesis of preeclampsia with miR-19a by measuring the expression of miR-19a in normal pregnancy and preeclampsia placenta. Methods: 10 cases of severe preeclampsia (experimental group) and 10 normal placenta maternal placenta (control) were collected, fluorescent Real-Time quantitative PCR method was used to detect the expression of miR-19a in two different groups. Results: miR-19a expression in patients with severe preeclampsia placenta increased significantly comparing with those in normal placenta. Conclusion: miR-19a expression increased significantly in preeclampsia placenta, and there exist an connection between high expression of miR-19a and the pathogenesis of preeclampsia.