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Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.) |
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| Authors: | Bruno Studer Torben Asp Ursula Frei Stephan Hentrup Helena Meally Aurélie Guillard Susanne Barth Hilde Muylle Isabel Roldán-Ruiz Philippe Barre Carole Koning-Boucoiran Gerda Uenk-Stunnenberg Oene Dolstra Leif Skøt Kirsten P Skøt Lesley B Turner Mervyn O Humphreys Roland Kölliker Niels Roulund Klaus K Nielsen Thomas Lübberstedt |
| Institution: | 1. Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Research Centre Flakkebjerg, University of Aarhus, Fors?gsvej 1, Slagelse, 4200, Denmark 2. Crops Research Centre Oak Park, TEAGASC, Carlow, Ireland 3. Plant Unit, Applied Genetics and Breeding, Institute for Agricultural and Fisheries Research (ILVO), Melle, Belgium 4. Unité de Génétique et d’Amélioration des Plantes Fourragères, Institut National de Recherche Agronomique (INRA), Lusignan, France 5. Plant Research International B.V. (PRI), Wageningen, The Netherlands 6. Plant Genetics and Breeding Department, Institute of Grassland and Environmental Research (IGER), Plas Gogerddan, Aberystwyth, UK 7. Agroscope Reckenholz-T?nikon Research Station (ART), Zurich, Switzerland 8. DLF-TRIFOLIUM, Dansk Plantefor?dling, Store Heddinge, Denmark |
| Abstract: | An expressed sequence tag (EST) library of the key grassland species perennial ryegrass (Lolium perenne L.) has been exploited as a resource for microsatellite marker development. Out of 955 simple sequence repeat (SSR) containing ESTs, 744 were used for primer design. Primer amplification was tested in eight genotypes of L. perenne and L. multiflorum representing (grand-) parents of four mapping populations and resulted in 464 successfully amplified EST-SSRs. Three hundred and six primer pairs successfully amplified products in the mapping population VrnA derived from two of the eight genotypes included in the original screening and revealed SSR polymorphisms for 143 ESTs. Here, we report on 464 EST-derived SSR primer sequences of perennial ryegrass established in laboratory assays, providing a dedicated tool for marker assisted breeding and comparative mapping within and among forage and turf grasses. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| Keywords: | Comparative mapping Expressed sequence tag (EST) Ryegrass (Lolium spp ) Simple sequence repeat (SSR) |
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