Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions |
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| Authors: | Linda Cerofolini Stefano Giuntini Letizia Barbieri Matteo Pennestri Anna Codina Marco Fragai Lucia Banci Enrico Luchinat Enrico Ravera |
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| Affiliation: | 1. Magnetic Resonance Center, University of Florence and Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine, Sesto Fiorentino, Italy;2. Department of Chemistry, Ugo Schiff, University of Florence, Sesto Fiorentino, Italy;3. Department of Experimental and Clinical Biomedical Sciences, Mario Serio, University of Florence, Florence, Italy;4. Bruker UK Limited, Banner Lane, Coventry, United Kingdom |
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| Abstract: | FlowNMR has the aim of continuously monitoring processes that occur in conditions that are not compatible with being carried out within a closed tube. However, it is sample intensive and not suitable for samples, such as proteins or living cells, that are often available in limited volumes and possibly low concentrations. We here propose a dialysis-based modification of a commercial flowNMR setup that allows for recycling the medium while confining the sample (proteins and cells) within the active volume of the tube. This approach is demonstrated in the specific cases of in-cell NMR and protein-based ligand studies. |
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| Keywords: | Corresponding author |
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