Ribosome degradation and the degradation products in starved Escherichia coli: I. Comparison of the degradation rate and of the nucleotide pool between Escherichia coli B and Q-13 strains in phosphate deficiency |
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| Affiliation: | 1. Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Louisville School of Medicine, Louisville, Kentucky;6. Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, Kentucky;11. Hepatobiology and Toxicology Program, University of Louisville School of Medicine, Louisville, Kentucky;12. University of Louisville Alcohol Research Center, University of Louisville School of Medicine, Louisville, Kentucky;2. College of Life Science, Jilin University, Changchun, China;3. National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland;4. National Institute on Aging, Baltimore, Maryland;5. Division of Gastroenterology, Department of Pediatrics, University of California San Diego, San Diego, California;7. Robley Rex Veterans Medical Center, Louisville, Kentucky;1. School of Pharmacy, Jiangsu University, Zhenjiang 212013, PR China;2. Affiliated Hospital of Jiangsu University, Zhenjiang 212001, PR China;3. Medical School, Shanghai University, Shanghai 200444, PR China;1. School of Physics and Astronomy, University of Minnesota, Minneapolis, MN 55455, USA;2. Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA;3. Benson Hill Biosystems, St. Louis, MO 63132, USA;1. Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, New South Wales, Australia;2. Division of Microbiology, Prince Sultan Military Medical City, Riyadh, Saudi Arabia;3. Antibiotic Resistance and Mobile Elements Group (ARMEG), Microbiology and Infectious Diseases Unit, School of Medicine, University of Western Sydney, New South Wales, Australia;4. Department of Microbiology and Infectious Diseases, Sydney South-West Pathology Service – Liverpool, New South Wales, Australia;5. Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim, Saudi Arabia;6. Whiteley Corporation, Tomago, Newcastle, NSW, Australia;1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan;2. Isotope Science Center, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan;3. Institute of Medical Mycology, Teikyo University, Otsuka 359, Hachiojishi, Tokyo, 192-0395, Japan;4. Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, Japan;1. Department of Clinical Chemistry and Clinical Pharmacology, University Hospital Bonn, Bonn, Germany;2. Axolabs GmbH, Fritz-Hornschuch-Strasse 9, 95326 Kulmbach, Germany;3. Institute of Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn, Bonn, Germany;4. Miltenyi Biotech, Biomedicine Division, Bergisch Gladbach, Germany;5. Department of Pediatrics and Adolescent Medicine, Division of Pediatric Neurology, University Medical Center Göttingen, Georg August University, Göttingen, Germany;6. German Center for Infection Research (DZIF), partner site Bonn-Cologne, Cologne, Germany |
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| Abstract: | The differences in ribosomal degradation and the fate of degradation products between Escherichia coli wild-type (B) and a ribonuclease less mutant (Q-13) during phosphate starvation were compared. The degradation rate of 32P-labeled ribosomes was found to be of the same order at the early stage but to differ at the later stage of deficiency between Escherichia coli B strain and Q-13 strain. The degradation in Q-13 nearly stopped after 6-h starvation, while it continued for more than 10 h in B strain, resulting in more than 80% degradation of 32P-prelabeled ribosomes.In the acid-soluble 32P pool of Q-13 strain, there was an increase in the nucleoside monophosphates but no increase and a continuous decrease in nucleoside di-and triphosphates, respectively. Q-13 contained no nucleoside 2′,3′-cyclic phosphate or nucleoside 3′-phosphate and little oligonucleotide, all of which had been detected in B strain during this stage. From these facts it was suggested that ribosomal RNA degradation in Escherichia coli Q-13 is mainly caused by ribonuclease II in vivo. |
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