General Information |
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| Authors: | Jin Wang Kai-Jing Zuo Jie Qin Lida Zhang Lan Su Junrong Liu Hua Ling Jing-Ya Zhao You-Fang Cao Ke-Xuan Tang |
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| Institution: | (1) Plant Genomics Research center, Mianyang Normal College, Mianyang, Sichuan, 621000, China;(2) Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, School of Life Science and Technology, Shanghai Jiaotong University, Shanghai, 200030, China;(3) Environmental Sciences Department, School of Chemical Engineering, Tsinghua University, 100800 Beijing, China |
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| Abstract: | A novel cDNA clone encoding a COR413-like gene was isolated by suppression subtraction hybridization and cDNA library screening from sea-island cotton (Gossypium barbadense). This gene (designated as GbCOR413, Accession number: AY761065) has a total length of 893 bp with an open reading frame of 600 bp, encoding a predicated polypeptide
of 200 amino acids with a molecular weight of 22.74 kDa and a predicated pI of 9.2. Bioinformatics analyses revealed that this gene belonged to a novel stress-regulated multi-spanning transmembrane
protein family without signal peptide. By means of semi-quantities RT-PCR analysis, the expression of GbCOR413 under short-term cold treatment at 4°C, water submergence and abscic acid treatment was investigated. Our studies suggested
that the cloned gene was a new member of COR gene family which was slowly responsive to cold stress in cotton.
Jin Wang and Kai-Jing Zuo are co-first authors of this paper. |
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| Keywords: | Gossypium barbadense COR413 Environmental stress Bioinformatics analyses |
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