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Steric hindrance effects on surface reactions: applications to BIAcore
Authors:David A. Edwards
Affiliation:(1) Department of Mathematical Sciences, University of Delaware, Newark, DE 19716-2553, USA
Abstract:Because surface-volume reactions occur in many biological and industrial processes, understanding the rate of such reactions is important. The BIAcore surface plasmon resonance (SPR) biosensor for measuring rate constants has such a geometry. Though several models of the BIAcore have been presented, few take into account that large ligand molecules can block multiple receptor sites, thus skewing the sensogram data. In this paper some general mathematical principles are stated for handling this phenomenon, and a surface-reaction model is presented explicitly. An integro-partial differential equation results, which can be simplified greatly using perturbation techniques, yielding linear and nonlinear integrodifferential equations. Explicit and asymptotic solutions are constructed for cases motivated by experimental design. The general analysis can provide insight into surface-volume reactions occurring in various contexts. In particular, the steric hindrance effect can be quantified with a single dimensionless parameter. This work was supported in part by NIGMS Grant 1R01GM067244-01.
Keywords:Biomolecular reactions  Rate constants  Asymptotics  Integro-differential equations  Steric hindrance effects  BIAcore
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