| Institution: | 1.Institute of Biochemistry, Faculty of Medicine,Justus-Liebig-University,Giessen,Germany;2.Department II of Internal Medicine,Center for Molecular Medicine Cologne, University Cologne,Cologne,Germany;3.Institute of Reproductive Biology,Leibniz Institute for Farm Animal Biology (FBN),Dummerstorf,Germany;4.Institute of Genome Biology,Leibniz Institute for Farm Animal Biology (FBN),Dummerstorf,Germany;5.Excellence Cluster Cardio Pulmonary System (ECCPS),Giessen,Germany |
| Abstract: | BackgroundSialic acids represent common terminal residues on numerous mammalian glycoconjugates, thereby influencing e.g. lumen formation in developing blood vessels. Interestingly, besides monosialylated also polysialylated glycoconjugates are produced by endothelial cells. Polysialic acid (polySia) is formed in several organs during embryonal and postnatal development influencing, for instance, cell migration processes. Furthermore, the function of cytokines like basic fibroblast growth factor (bFGF) is modulated by polySia.ResultsIn this study, we demonstrated that human umbilical vein endothelial cells (HUVEC) also secrete polysialylated glycoconjugates. Furthermore, an interaction between polySia and vascular endothelial growth factor (VEGF) was observed. VEGF modulates like bFGF the migration of HUVEC. Since both growth factors interact with polySia, we examined, if polySia modulates the migration of HUVEC. To this end scratch assays were performed showing that the migration of HUVEC is stimulated, when polySia was degraded.ConclusionsSince polySia can interact with bFGF as well as VEGF and the degradation of polySia resulted in an increased cell migration capacity in the applied scratch assay, we propose that polySia may trap these growth factors influencing their biological activity. Thus, polySia might also contribute to the fine regulation of physiological processes in endothelial cells. |
