Cloning and sequencing of beta toxin gene of Clostridium perfringens type C |
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| Authors: | Goswami P P Girish K S Chaudhuri P Tiwari V Akare S J Harbola P C |
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| Institution: | National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India. |
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| Abstract: | A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfingens. |
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