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beta-Amyloid deposition and prion infection in adult primary brain cell long-term culture model |
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| Authors: | Yun Seong-Wook Kouznetsova Elena Nitschke Cindy Heinitz Katrin Schliebs Reinhard Gerlach Manfred Riederer Peter Klein Michael A |
| Affiliation: | a Clinical Neurochemistry and NPF Center of Excellence Laboratories, Clinic of Psychiatry and Psychotherapy, University of Wurzburg, 97080 Wurzburg, Germany b Department of Neurochemistry, Paul Flechsig Institute for Brain Research, University of Leipzig, 04109 Leipzig, Germany c Institute of Virology and Immunobiology, University of Wurzburg, 97078 Wurzburg, Germany d Clinic of Child and Adolescent Psychiatry and Psychotherapy, University of Wurzburg, 97080 Wurzburg, Germany |
| Abstract: | We report an in vitro model of the adult central nervous system produced by culturing primary brain cells isolated from adult mice for periods longer than 4 months. We applied this novel cell culture method to model progressive neurodegenerative diseases. After long-term culture of adult primary brain cells prepared from Alzheimer’s disease and prion disease mouse models, we observed β-amyloid deposition and prion infection in primary cell cultures in vitro. |
| Keywords: | Alzheimer&rsquo s disease β-Amyloid Brain Cell culture Primary cell Prion |
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