The potential use of silicon compounds as oxygen carriers for free and immobilized cells containing l-amino acid oxidase |
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| Authors: | Andreas Leonhardt Estera Szwajcer Klaus Mosbach |
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| Institution: | (1) Institut für Makromolekulare Chemie, Universität Freiburg, Stefan-Meier-Strasse 31, D-7800 Freiburg, Federal Republic of Germany;(2) Pure and Applied Biochemistry, Chemical Center, University of Lund, P.O. Box 740, S-220 07 Lund, Sweden;(3) Institute of Biotechnology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland |
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| Abstract: | Summary The relatively low solubility of oxygen in water presents a problem in particular when immobilized cells are used or enzymes are applied for oxygen dependent reactions. The other main purpose is the requirement of oxygen for the increase of biomass. In this investigation the usefulness of silicon emulsions as oxygen carriers is demonstrated.In case of l-amino acid oxidase activity of immobilized cells, an increase by a factor of four was found in the presence of silicon emulsions. Likewise, growth medium enriched with silicon emulsions showed a significantly increased growth of cells inside alginate beads compared to normal growth medium. |
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