汉坦病毒HTN261株S基因片段的核苷酸序列测定及分析

黑龙江省是肾综合征因热（HFRS）的重疫区。近年来HFRS年的发病人数曾超过万人。流行病学和血清学研究表明黑龙江省HFRS疫区主要是姬鼠型，但目前尚缺乏病毒的分子生物学资料。我们对从疫区捕获的宿主动物－黑线姬鼠肺中分离的汉坦病毒HTN261株的S基因片段的全基因序列进测定和初步分析。结果如下，HTN261株的S基因片段的全序列长为1697nt。只有一个主要的编码N蛋白的ORF，起始位置为第37nt，终止于1326nt，编码的蛋白长为429aa。没有发现存在ORF2。HTN261株的S基因片段核苷酸序列与HGTN型中的病毒株的同源性很高，而与汉坦病毒其他型的同源民生较差，从种系发生树分析来看，HNT261株归结于汉坦病毒的HTN型。在HTN型之内，HTN261株和HTN76－118株在一个分枝内，就其核苷酸和蛋白的同源性说，HT N261株和HTN76－118株的同源性分别是89％（全S基因）和98％（蛋白）。而与中国境内发现的其他汉坦病毒株Z10，HU，Chen4,NC167等基因和蛋白的同源性相对较差，汉坦病毒除具有其宿主的依赖性外，还具有其地理的簇集性。HTN261株和HTN76－118株之间S基因和N蛋白序列的变异性的差异分别为11％和2％，表明HTN261株和HTN76－118株还有不同，可能是不同的亚型。不过，尚有待于进一步研究证明。

Complete Nucleotide Sequennce of the S Genome Segment of Hantavirus HTN261 Strain Isolated in the Northeast China

To determine the complete nucleotide sequence of the S genome segment of HTN261 virus strain which was isolated from the striped field mouse (Apodemus agraius) in Heilongjiang province of the Northeast China, the complete nucleotide sequence of the S genome segment of HTN261 virus strain was determined with two clones, which were obtained using RT PCR, and by linking two overlapping fragments into PUC19 vector respectively. The complete nucleotide sequence of the S genome segment of HTN261 virus strain was 1697 nucleotides in length and contained one ORF encoding the N protein starts at position 37 and ends at position 1326. The length of predicted protein, 429 amino acids, is identical to the N protein of HTN, DOB, SEO types of hantaviruses. Comparison of S segment sequence of HTN261 strain with the corresponding sequences of other hantaviruses showed that the identities of HTN261 strain with HTN76 118 strain were 89% (complete nucleotide sequence) andd 98% (amino acid sequence). Phylogenetic analysis showed that HTN261 strain with HTN76 118 strain form one lineage within the clade containing hantaviruses of HTN type. But the identities of HTN261 strain with HTN76 118 strain differ by 11% in complete nucleotide sequence of S segment and 2% in amino acid sequence of N protein. HTN261 strain belongs to hantavirus HTN type and may be a new subtype.