乙型肝炎病毒全S基因疫苗诱导小鼠的特异性免疫应答

乙型肝炎病毒 (ＨＢＶ)感染是我国常见病及多发病。ＨＢＶ难以清除的原因之一就是机体的免疫功能障碍。目前虽然基因重组ＨＢＶ表面抗原 (ＨＢｓＡｇ)疫苗预防ＨＢＶ感染取得了较好的效果 ,但基因重组ＨＢｓＡｇ疫苗主要能诱导特异性体液免疫 ,不能刺激机体的细胞免疫应答。近年来发现基因疫苗可诱导机体产生细胞及体液免疫反应 ,特别是诱导细胞免疫反应的能力优于蛋白、多肽类疫苗 ,更适应于慢性病毒感染的预防与治疗1,2 ] 。为了探讨应用ＨＢＶ基因疫苗预防ＨＢＶ感染的可能性 ,本文构建了ＨＢＶ全Ｓ基因和ＨＢｓＡｇ基因疫苗 ,观察和比…

Specific Immune Responses Against Hepatitis B Virus Following Immunization with the DNA Vaccine Containing HBV pan-S Gene in Mice

In order to know the specific immume responses after immunization of HBV DNA vaccines and what kind differences of immune responses following the HBV pan-S gene and HBsAg gene vaccination,the DNA vaccines,both pcDNA3-PS and pcDNA3-HBsAg,in which HBV pan-S gene and HBsAg gene sequences were inserted into downstream of CMV promoter in pcDNA3,respectively,were injected intramuscularly into BALB/C mice.Specific humoral and cellular immune responses were detected after Vaccination.The serum anti-HBs were detected in all of the pcDNA3-PS and the pcDNA3-HBsAg immunized mice,but none in control mice.However,there were no significantly differences of serums anti-HBs level between the pcDNA3-PS and pcDNA3-HBsAg immunized mice.Serum levels of interleukine 2 and interferon gamma,were also significantly higher in pcDNA3-PS and pcDNA3-HBsAg immunized mice than that in controls.It was indicated that the good responses of cellular and humoral immunity can be induced by injection of pbDNA3-PS and pcDNA3-HBsAg in BALB/C mice.No any evidence was showed that HBV pan-S gene vaccine could enhance the immunity of HBsAg gene vaccine.