Stimulation of phospholipase D activity and indication of acetylcholine synthesis by oleate in rat brain synaptosomal preparations |
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| Authors: | H Hattori J N Kanfer R Massarelli |
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| Institution: | (1) Department of Biochemistry, University of Manitoba, Winnipeg, Canada;(2) Centre de Neurochimie du CNRS, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France |
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| Abstract: | It had been previously demonstrated that the oleate activation of synaptosomal membrane phospholipase D liberated choline which was available for acetylcholine formation. The present investigations were undertaken to determine if oleate might have an effect on choline uptake by synaptosomes. It was observed that oleate interfered with choline uptake when incubations were carried out at 37°C but uptake was stimulated at 3°C. Oleate was the most effective fatty acid of several tested. Preliminary observations suggest the presence of a membranous form of choline acetyltransferase. |
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| Keywords: | Choline uptake phospholipase D oleate synaptosomes |
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